awesome-nextflow
by nextflow-io
A curated list of nextflow based pipelines
AI summary
Biological workflows
A curated collection of reusable workflow pipelines for various biological analysis tasks.
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155 links in 11 sections, with live GitHub stats.activeno commit in 2y
Example pipelines
cbcrg/ampa-nf
Automated prediction of protein antimicrobial regions
cbcrg/mta-nf
A method for best alignment of evaluation trees
nextflow-io/rnaseq-nf
A basic pipeline for quantification of RNA-seq data using Salmon
nextflow-io/rnatoy
A basic RNA-seq pipeline
nextflow-io/elixir-workshop-21
A basic pipeline with QC and alignment
Featured pipelines
AndersenLab/cegwas2-nf
GWA mapping with C. elegans
alesssia/YAMP
Yet Another Metagenomic Pipeline
anvlasova/FA-nf
Functional annotation pipeline for proteins from non-model organisms
biocorecrg/allele_specific_RNAseq
Allele-specific RNA-seq pipeline
bactopia/bactopia
A flexible pipeline for complete analysis of bacterial genomes
biocorecrg/ExOrthist
ExOrthist is a Nextflow based pipeline to infer exon orthology groups at all evolutionary distances
biocorecrg/indrop
Single cell transcriptome analysis pipeline based on DropEst
biocorecrg/master_of_pores
Nextflow pipeline for analysis of Nanopore reads
biocorecrg/MOP2
DSL2 version of master of pores. Faster and better
biocorecrg/transcriptome_assembly
De novo transcriptome assembly and annotations workflow based on trinity / transdecoder
biocorecrg/vectorQC
A Nextflow pipeline for assembling and annotating vectors
brwnj/smoove-nf
Smoove workflow for Structural Variant calling and Quality Control
cbcrg/kallisto-nf
Nextflow implementation of Kallisto & Sleuth RNA-Seq Tools
cbcrg/piper-nf
RNA mapping pipeline
cbcrg/unistrap
Reliability measure of inferred phylogenetic trees
crickbabs/BABS-aDNASeq
An ancient DNA nextflow analysis pipeline
CRG-CNAG/CalliNGS-NF
Variant Calling Analysis with RNA-Seq data based on GATK best practices
Dowell-Lab/ChIP-Flow
Nextflow pipeline for ChIP-seq analysis
Dowell-Lab/RNAseq-Flow
Nextflow pipeline for processing RNA-seq data
Dowell-Lab/Nascent-Flow
Nascent Transcription Processing Pipeline
FredHutch/reproducible-workflows
Reproducible Workflows, curated at the Fred Hutch
guigolab/chip-nf
Automated ChIP-seq pipeline
guigolab/grape-nf
Automated RNA-seq pipeline
guigolab/ipsa-nf
Integrative Pipeline for Splicing Analyses
guigolab/FA-nf
Functional annotation pipeline for proteins from non-model organisms
h3abionet/h3abionet16S
Integrated pipeline for 16S rDNA diversity analysis
IARC/needlestack
Multi-sample somatic variant caller
IARC/gatk4-GenotypeGVCFs-nf
Joint calling of gVCF, following GATK4 Best Practices
IARC/gatk4-HaplotypeCaller-nf
GATK4 HaplotypeCaller step, in gVCF mode, first step for subsequent whole cohort Joint Genotyping
icgc-argo/sanger-wgs-variant-calling
Sanger WGS Variant Caller
jdidion/atropos
NGS read trimming tool that is specific, sensitive, and speedy
labsyspharm/mcmicro
End-to-end pipeline for processing multiplexed whole slide imaging and tissue microarrays
- loosolab/TOuCAN
Targeted chrOmatin Capture ANalysis
metagenomics/MeRaGENE
Metagenomics rapid gene identification pipeline
mirnylab/distiller-nf
Modular Hi-C mapping pipeline
montilab/pipeliner
Framework for the definition of sequencing data processing workflows
NCBI-Hackathons/ATACFlow
ATAC-seq pipeline wrapped in NextFlow
NCSA/GenomicsCortextVarNextflow
Structural Variant Calling with Cortex-Var
nf-core/chipseq
Chromatin immunoprecipitation (ChIP-seq) peak-calling, QC and differential analysis pipeline
nf-core/eager
A fully reproducible and state of the art ancient DNA analysis pipeline
nf-core/hlatyping
Precision HLA typing from next-generation sequencing data
nf-core/lncpipe
Analysis of long non-coding RNAs from RNA-seq datasets
nf-core/mag
Assembly and binning of metagenomes
nf-core/methylseq
Methylation (Bisulfite-Sequencing) analysis pipeline using Bismark or bwa-meth + MethylDackel
nf-core/pangenome
Renders a collection of sequences into a pangenome graph
nf-core/rnaseq
RNA sequencing analysis pipeline using STAR, HISAT2 and Salmon with gene counts and quality control
nf-core/rnafusion
RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes
nf-core/sarek
Analysis pipeline to detect germline or somatic variants from WGS / targeted sequencing
nf-core/smrnaseq
A small-RNA sequencing analysis pipeline
nf-core/vipr
Assembly and intrahost / low-frequency variant calling for viral samples
nmdp-bioinformatics/flow
Consensus assembly and variant calling workflow
robsyme/nf-repeatmasking
Workflow for automatic repeat detection, classification and masking
qbicsoftware/icgc-featurecounts
Pipeline to run featureCounts on RNAseq BAM files
sanger-pathogens/companion
Eukaryotic genome annotation pipeline
ZuberLab/crispr-process-nf
Process CRISPR and shRNA functional genetic screening data
ZuberLab/mageck-nf
Statistical Analysis of multiplexed CRISPR / shRNA Screens using MAGeCK
Covid-19 related projects
biocorecrg/master_of_pores
Nextflow pipeline for analysis of Nanopore data from direct RNA sequencing (see )
BU-ISCIII/SARS-Cov2_analysis
SARS-Cov2 bioinformatics analysis
connor-lab/ncov2019-artic-nf
A Nextflow pipeline for running the ARTIC network's fieldbioinformatics tools with a focus on ncov2019
heuermh/sars-cov-2
Transform SARS-CoV-2 annotated genomes into sequences and features in format
jnoms/virID
Viral Identification and Discovery - A viral characterization pipeline built in Nextflow
- vtilloy/aspicov
Automated and Standardized Pipeline for Identification of SARS-Cov2 nucleotidic Variants
Other pipelines
abreschi/ShortRNA-nf
Pipeline for processing shortRNA-seq data
andremrsantos/next-pipes-lghm
Eukaryotic genome annotation pipeline
cbcrg/grape-nf
Yet another RNA-Seq pipeline (deprecated)
cerebis/meta-sweeper
Parametric sweep of simulated microbial communities and metagenomic sequencing
chrisquince/DESMAN
De novo Extraction of Strains from MetAgeNomes
evanfloden/concTree
Creates consensus trees using alignment uncertainty from many MSAs
evanfloden/lncRNA-Annotation-nf
lncRNA Annotation Pipeline based on STAR, Cufflinks and FEELnc
evanfloden/paraMSA
Multiple sequence alignments for phylogenetic trees and bootstrap support values
evanfloden/tuxedo-nf
Nextflow implementation of the Tuxedo Suite of Tools
evanfloden/vast-tools-nf
Nextflow Implementation of VAST-TOOLS for profiling alternative splicing events in RNA-Seq data
h3abionet/chipimputation
Imputation workflow developed as part of the H3 Africa BioNet Hackathon
h3abionet/h3agwas
GWAS pipeline developed as part of the H3 Africa BioNet Hackathon
holtgrewe/ngs_pipelines
Nextflow-based pipelines for typical NGS processing tasks
IARCbioinfo/bametrics-nf
Compute average metrics from reads that overlap a given set of positions
IARCbioinfo/mpileup-nf
Coverage computation with samtools mpileup
jdidion/rna-quick
Rapid analysis of gene and transcript quantification and differential expression
joshua-d-campbell/nf-GATK_Exome_Preprocess
GATK best practice guide to preprocess whole exome sequencing (WES) data
CDCgov/mycosnp-nf
Portable workflow for performing whole genome sequencing analysis of fungal organisms
NationalGenomicsInfrastructure/icing
HLA typing from OxfordNanopore reads
NBISweden/wgs-structvar
Genome Sequenceing Structural Variation Pipelines
MHH-RCUG/nf_wochenende
A metagenomic (and genomic) alignment and normalization pipeline for long and short reads
ODiogoSilva/innuca-nf
A nextflow implementation of INNUENDO quality control of reads, de novo assembly and contigs quality assessment
CDCgov/PHoeNIx
A short-read pipeline for healthcare-associated and antimicrobial resistant pathogens
PlantandFoodResearch/VariantAnalysis
Bioinformatic variant calling pipeline
robsyme/nextflow-annotate
Fungal genome annotation workflow
robsyme/workflows-proteogenomics
Versioned, shareable and replicable workflows for proteogenomics
CDCgov/SC2CLIA
Pipeline for Clinical Laboratories Improvements Amendments (CLIA) compliant variant calling and spike protein substitution prediction with additional tools for quality control
- snewhouse/ngs_nextflow
Alignment, Variant Calling, GATK Best Practices
wittelab/orchid
Novel management, annotation, and machine learning framework for analyzing cancer mutations
Tutorials
Nextflow patterns
A collection of common Nextflow implementation patterns
Nextflow demos
Example Nextflow pipelines and programming techniques (by Stephen Kelly)
Pipelines collections
Modules collections
biocorecrg/BioNextflow
Repository to host DSL2 based tool-specific sub-workflows
nf-core/modules
Repository to host tool-specific module files for the Nextflow DSL2 community
UMCUGenetics/NextflowModules
UMCU Genetics Nextflow modules
Presentations
- rsuchecki.github.io
Reproducible and Reusable publication and analysis workflow by
- CIC Show-and-Tell
Nice Nextflow presentation by
Video
- Nextflow: Scalable, Shareable & Reproducible workflows
Awesome intro by Rad Suchecki
Syntax highlighting
Other links
Nextflow Hackathon '17
Projects repositorty of the Nextflow Hackathon held in Barcelona, Sept 2017
- Nextflow Workbench
Workbench that make it easier to write data analysis workflows with Nextflow
nf-core/tools
Helper tools for the nf-core community, linting, template
Make to Nextflow
Converts Makefile to Nextflow
NCBI-Hackathons/SPeW
A discussion comparting different workflow frameworks including Nextflow
- NGS workflow
Excellent blog post comparing Make/Snakemake/Nextflow by Julian Mazzitelli
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